№17926728[Quote]
RAAAAAAAPE
№17926735[Quote]
White people be like:
№17926746[Quote]
Xhe dresses like that to please BBC
№17926757[Quote]
They're cute until you reach post-nut clarity and you see them irl.
№17926805[Quote]
>>17926778Why did you add random dots and retarded emojies you fucking mongoloid jeeta.
Even your shithole of an India has trans people xDDD
№17926814[Quote]
>>17926805its a soyQUOTE
read ops image FAGGOT
№17926889[Quote]
https://mudae.net/character/3644215/Kurumi%20Nanasebased off of this bullshit
ignoring the troon aspects this is literally direct ai slawp of this fuggen tranime character (which is a minor)
Duct tapes, they are making a minor troon out
№17926912[Quote]
>>17926889this schizo is back
№17926918[Quote]
>>17926912don't even try, i have a scropt that filter every line above 10
don't even try, i have a scropt that filter every line above 10
№17926989[Quote]
SuperSAGE is a variant of the Serial Analysis of Gene Expression (SAGE) technology, based on counting transcripts by sequencing analysis of short sequence tags. In SuperSAGE, 26 bp tags are extracted from cDNA using the Type III restriction endonuclease EcoP15I. The use of a longer tag size in SuperSAGE allows a secure tag-to-gene annotation in any eukaryotic organism. We have succeeded in combining SuperSAGE and high-throughput sequencing technology (Now- or Next-Generation Sequencing, NGS) in an approach we call High-throughput SuperSAGE (HT-SuperSAGE). This approach allows deep transcriptome analysis and multiplexing, while reducing time, cost, and effort for the analysis. In this chapter, we present the detailed HT-SuperSAGE protocol for both the Illumina Genome Analyzer and also the AppliedBiosystems SOLiD sequencer.